DS-spil vil forblive regionsfrie på DSi og du kan derfor bruge dem på en DSi fra hvilken som helst region du har lyst til.
Nintendo udtaler sig her om DSi og regionslåsningen:
DSi software (software that is only compatible with DSi) is region locked, eg: European DSi software can only be played on European DSi consoles... Nintendo DS software however is region free so you can play any DS software on DSi from any region. You can also browse the Internet on your DSi wherever you are in the world and exchange your photos with friends from around the world... DSi is region locked because DSi embeds net communication functionality within itself and we are intending to provide net services specifically tailored for each region. Also because we are including parental control functionality for Nintendo DSi and each region has its unique age limit made by different independent.
For at opsummere citatet bliver DSi blandt andet regionslåst fordi muligheden for at gå på nettet vil være en integreret del af DSi. Nintendo ønsker derfor at tilbyde hver region internet services tilpasset lige netop den region DSi'en er låst til.
Derudover bliver der også inkluderet en forældrekontrol funktion (bruges til at bestemme, hvad ens børn må have adgang til) og hver region har sine egne aldersgrænser.